Themen: Immunodefficiencies (Halin) & Immunogenicity (Neri)

H: Was ist Immunodefficiency?
A: Immunsystem kann nicht richtig auf Pathogene reagieren.
H: primary vs aquired ID
A: primary genetisch, merkt man schon früh als kind, aquired durch HIV, chemotherapie.
H: was ist bei chemo das problem?
A: killing of Neurtophils, B and T cell is not that bad
H: Why?
A: Neutrophils are part of innate IS, needed as first-line defense.
H: What else?
A: TH17 defense.
H: Was für genetische ID gibt es?
A: zBsp. betreffend AK, XLA, habe XLA erklärt
H: Was haben Betroffene für Probleme?
A: Opportunistische Infekte, Bakterien, Viren
H: Warum Viren?
A: AK gebraucht um zBsp andocken von Virus an Zelle zu unterbinden (SARS-CoV-2 und Spike)
H: ist XLA schlimm?
A: gibt schlimmere, XSCID, weil pathogen-frei und brauchen Knochenmark-Transplantation.

N: What does immunogenity mean?
A: Answer of IS to drug, production of AB
N: How would you detect AB against IFN-beta?
A: ELISA, explain how it works
N: Would that also work for Humira?
A: No, because Humira and Humira-AB are both fully human -> rabbit-anti-human-AB would bind to both
N: What are they doing instead?
A: Biacore
N: No, but that would have been a much better idea
A: Displacement assay with rabbit-AB
N: What is the problem with this method?
A: didnt know, said something wrong
N: Well, what part of AB is most immunogenic?
A: variable region
N: Explained to me why this matters, but I didnt understand
N: Do you know a product on the market which is modified and is still not immunogenic?
A: Insulin
N: What do you do when the patient makes Anti-Humira-ABs?
A: Switch to a different product.