Had the same questions as the person above me.
Halin: Distribution of antibodies
IgG in whole body, can permeate through placenta, IgE is bound to IgE receptor, IgM pentamer and cannot permeate, IgA dimer, is distributed into breast milk, IgD bound on B cells
How exactly is IgG transported through placenta? Didn't know that, explained FcRn recycling, apparantly it is the exact same mechanism (histidine protonation before binding to FcRn)
Which cells express FcRn? I had to guess, the right answer was endothelial cells.
What happens with IgA in the baby? Only in GI tract, helps with gut infections. I said it alsi helps with respiratory infections to a low extent, Halin told me she never heard of this.
I was a bit disappointed that Halin asked something which wasn't really a topic of TP but rather Immunology.
Neri: History of therapeutic proteins
Panicked in the beginning because I didn't learn this script at all. Said that insulin was the first protein to be recombinantly produced.
Which company? Genentech, didn't know this. Luckily he then asked more about insulin.
Chemical structure? A and B chain, connected with disulfide bonds.
Expression systems and pros/cons? E. Coli, Yeast, mammalian
Categories of different therapeutic proteins and one example? Growth factors, coagulation factors, vaccines, antibodies
Indications of different antibodies? Focused on anti-inflammation, anti-cancer, cardiovascular, anti-viral. He wanted to hear more but I couldn't focus :`) He told me ophthalmic, cholesterine antibodies etc.
How can antibodies be chemically modified? Better affinity for FcRn, PEGylation, only use Fab or scFv fragment. That's not what he meant, he wanted to hear more about conjugation.
They were both very nice and helped me if I didn't know something.
Good luck! :)